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KMID : 1094720130180040787
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Biotechnology and Bioprocess Engineering
2013 Volume.18 No. 4 p.787 ~ p.795
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Immobilization of Rhizopus oryzae lipase on magnetic Fe3O4-chitosan beads and its potential in phenolic acids ester synthesis
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Kumar Vinod
Jahan Firdaus
Raghuwanshi Shailendra
Mahajan Richi V.
Saxena Rajendra Kumar
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Abstract
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A novel and simple method was developed for the preparation of magnetic Fe3O4 nanoparticles by chemical co-precipitation method and subsequent coating with 3-aminopropyltrimethoxysilane (APTMS) through silanization process. Magnetic Fe3O4-chitosan particles were prepared by the suspension cross-linking and covalent technique to be used in the application of magnetic carrier technology. The synthesized immobilization supports were characterized by scanning electron microscopy (SEM), thermogravimetric analysis (TGA) and X-ray diffraction (XRD). Using glutaraldehyde as the coupling agent, the lipase from R. oryzae was successfully immobilized onto the functionalized magnetic Fe3O4-chitosan beads. The results showed that 86.60% of R. oryzae lipase was bound on the synthesized immobilization support. This immobilized lipase was successfully used for the esterification of phenolic acid which resulted in esterification of phenolic acid in isooctane solvent reaction system for 8 consecutive cycles (totally 384 h), 72.6% of its initial activity was retained, indicating a high stability in pharmaceutical and industrial applications.
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KEYWORD
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magnetic Fe3O4 nanoparticles, Fe3O4-chitosan beads, R. oryzae lipase, ethyl ferulate
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